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Heng Li's blog

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Posts

Short RNA-seq read alignment with minimap2

Why is bwa-aln used for ancient DNA reads?

Where did BWA come from?

On the definition of pangenome

What high-performance language to learn?

Random open syncmers

A few suggestions for creating command line interfaces

Introducing dual assembly

Remapping an aligned BAM

Designing a command-line interface

An FM-index of 400k SARS-CoV-2 genomes

Concepts in phased assemblies

SNP vs SNV

Minigraph as a multi-assembly SV caller

Evaluating collapsed misassembly with asmgene

Base quality scores are essential to short read variant calling

Format, quality binning and file size

Fast high-level programming languages

auN: a new metric to measure assembly contiguity

On a reference pan-genome model (Part II)

On a reference pan-genome model

How much does developement time matter?

On maintaining bioinformatics software

SAM/BAM/samtools is 10 years old

On the definition of sequence identity

Seqtk: code walkthrough

On the MPEG-G alignment format

Minimap2 and the future of BWA

The history the MD tag and the CIGAR X operator

Immature thoughts on assembly De Bruijn graphs

Which human reference genome to use?

On NovaSeq Base Quality

Bioconda: a capable bio-software package manager

A reimplementation of symmetric DUST

A few comments on GraphMap

My thoughts on sharing genotype and phenotype data

A few hours with docker

The unary representation of variants

The problems with the VCF model

Correcting Illumina sequencing errors: extended background

The early history of the SAM/BAM format

BWA-MEM for long error-prone reads

On HiSeq X10 Base Quality

On the graphical representation of sequences

First update on GFA

Alternatives to PSMC

A proposal of the Grapical Fragment Assembly format

On the trend of disk-based algorithms

About static linking

Abreak: evaluating de novo assemblies

Random access to zlib compressed files

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